FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI05.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI05.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28917858
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC576010.1991883354569346No Hit
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC517260.17887216957770524No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA448210.1549941907868833No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA433700.14997653007356215No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT415570.14370704773500168No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT343340.11872940243361041No Hit
CTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAGCTCAACATACATACT299560.10358996852394807No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG297430.1028533994461139No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC213100.053.5394551
GTGGCGC211650.042.78393
TACAACC250300.039.9447252
TGGCGCA184650.038.827294
CTACAAC282150.036.8338241
GCGCACG197500.036.3513346
GGTGGCG297550.035.3675882
GGCGCAC231450.031.603595
CGCACGC234500.031.593621
GCACGCC263450.027.8415872
ACAACCA382400.026.3905333
GCGACAT77800.025.731147
TGCGACA77350.025.4152646
ACCAACC405800.024.8609896
GCGCCCG83900.023.68866
CACGCCT317300.023.0484493
ACGCCTG329250.022.2779624
CCAACCA468850.021.1031177
GCCTTAT194750.020.5170735
TGGCGCC97350.020.4819284
Produced by FastQC (version 0.11.3)