FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI06.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI06.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33061084
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT1090210.32975627780383726No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT854130.25834906078699654No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT772920.2337854378882435No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG758510.2294268391199756No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG710710.2149687529906763No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA678440.20520803250129366No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG673430.20369265569150727No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA669440.20248579871125824No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG642440.1943190973411519No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT636150.19241655839233826No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA636030.19238026194180444No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCT609120.1842407829095985No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT593940.17964928191707205No Hit
ATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCT556600.16835503639263613No Hit
GTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTCTCG538780.16296501348836595No Hit
CCAATTTGTAATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGTG536640.16231772678717976No Hit
CTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCA516910.15634998537857986No Hit
GTTTTTGGTAAACAGGCGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTT504430.15257515452306403No Hit
CGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCA490010.14821353105058502No Hit
GTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTC457370.13834089650538983No Hit
GGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTA455130.13766336276209212No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG448400.13562773682798784No Hit
CTCGTTTAGCCATTCATTCTAGTCCCTAATTAAGGAACAAGTGATTATGC432470.13080938301962514No Hit
ATAAGATATTAGTATTACTTTGACTTGTGAGTCTAGGTTAAAATCATTCG432170.1307186418932906No Hit
CCCAACCGAAATTTTTTAGTTCATATTTATTTTGTTTTAGCCCATTAGGT412970.12491120980788167No Hit
CTGATGGTAAGCTTAAAGTTGAACTGAAATTCTTTTTTGGGCAACCAGCT391470.11840809575390814No Hit
ATTAGGTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGG380330.11503857526268647No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC369270.11169325240515404No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTGCACCATTGGGAT348500.10541094175859449No Hit
TAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTTA339370.10264938681381408No Hit
GTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGA333790.10096160186399213No Hit
GTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTT331080.10014190702277033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTT79350.033.854141
GAGTCTT358800.030.6906244
GGAGTCT361950.030.6225053
AGTCTTG374850.029.6196965
TGGAGTC373950.028.9079862
GTCTTGG394150.028.0229026
CGATTTG73450.027.6508182
TCTTGGA446750.025.5612017
CTGGAGT616800.024.7266541
CTTGGAA466750.024.158728
CGCTATG67450.023.8747431
TTGGAAG491200.022.6778499
CGATGCG48600.022.0800823
CGCGGGT40600.021.8773571
CCCGTTT111000.021.468751
CGCTGTT507650.021.465481
CGCCGAG56600.020.2860031
CCCGAAT49200.019.2274651
TCGTTTG509050.019.1137352
GTTTAAC567550.018.970961
Produced by FastQC (version 0.11.3)