FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI06.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI06.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33061084
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC921710.2787900118459516No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA793890.24012824261902604No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA730480.22094859321612081No Hit
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC678140.20511729137495915No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT522350.1579954244694457No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT514740.15569362456476019No Hit
TGACAACCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCA509210.15402096313599398No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTCATGAATCAACCCGTCTA464180.14040072007318333No Hit
CATAACTAAACCCCCACATAAACTAAAACATTTAACTCAAAAAGTATTGG448800.1357487249964339No Hit
TTTCAGTTCAACTTTAAGCTTACCATCAGAACAACAAATCAAAATGTAAA444340.13439970691826075No Hit
CTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAGCTCAACATACATACT443110.13402766830028923No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG428330.12955715547620883No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTTTAGGTAGAGGTGAAAAGCC404230.12226761832733615No Hit
CTAAACCCCCACATAAACTAAAACATTTAACTCAAAAAGTATTGGAGAAA397570.12025316532270994No Hit
CCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAG389970.11795439012223556No Hit
CAATTATCGAATCATAGGTACTAACCCAACAATAGAATTACCTATCCCTA375180.113480852593944No Hit
CTTACACACACACTAATTCCACAAACCTCAATAAATTCCTATATTACAAA372230.11258856485165458No Hit
CCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCAACAATA359200.1086473752645255No Hit
CTACAATCACTTAACCATTGTAGGCTTAAAAGCAGCCATCAATAAAGAAA355260.10745564180533222No Hit
CTGGCCTTATAATTAATTGGAGGTAAGATTACACATGCAAACATCCATAA350740.1060884755018922No Hit
CTGTCTCTTACTTTCAATCAGTGAAATTGACCTTCCAGTGAAGAGGCTGG339270.10261913977170259No Hit
GCAAAATAGTGGGAAGATTTTTAGGTAGAGGTGAAAAGCCTATCGAGCTT336810.10187506253575956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC243100.058.7265551
TACAACC307800.048.9930842
CTACAAC338450.045.7905771
GTGGCGC236900.044.391983
GGTGGCG332750.038.5918962
TGGCGCA213350.037.8215684
ACAACCA456800.033.233023
GCGCACG250600.032.182856
CGCACGC302000.030.7806871
GCGACAT99100.029.5738777
GGCGCAC277300.029.5450025
ACCAACC540450.028.4065976
TGCGACA100900.028.4039976
GCGCCCG93800.027.7135356
GCACGCC347400.026.5837462
GGCCTTA238750.024.2399673
GCCTTAT236950.024.1814824
CCTTATA238250.024.1135755
CCAACAT582400.023.5785378
CCAACCA643850.023.3748687
Produced by FastQC (version 0.11.3)