FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI07.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI07.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38739216
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT899160.23210588464154772No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG859720.221924986814395No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG844240.21792903604450847No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT678140.17505258753816805No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT662750.17107986904019945No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA655870.16930389092024992No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA654820.1690328477478739No Hit
ATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCT646800.16696259418363035No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG634840.16387528338209012No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA586580.1514176229069788No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG574440.1482838475616027No Hit
GTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTCTCG565280.1459193185530652No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT564110.1456172990181319No Hit
GTTTTTGGTAAACAGGCGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTT548000.1414587223448198No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT522760.13494336075360946No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCT515630.13310284854499893No Hit
CTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCA495900.12800981826787616No Hit
GTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTC493050.12727412965714124No Hit
CTCCTTTGTCCTTTAGTTTCATAAAGGGTTTCGTAATGTTCTCTGGGAAA476790.12307683253063252No Hit
CGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCA436230.11260682198627871No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG432650.11168269383665379No Hit
CTGAAGATGGCGGTATATAGGCTGAATTAGCGAGAAGGGGTAAGGTAGAA416260.10745183898404138No Hit
CCAATTTGTAATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGTG411820.10630571356942278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTT89350.030.7107071
CGACGGA58100.023.6144711
GAGTCTT386850.023.494484
GGAGTCT402300.023.3619793
CGATTTG84000.023.234812
CGCGTTT26000.023.0518341
AGTCTTG409700.022.6567255
CGATGCG51350.022.5805513
GTCTTGG428950.022.0589686
TGGAGTC426150.021.5136972
CGCTGTT456850.020.2951451
CTGGAGT656350.020.2653871
CCCGTAT36100.019.8028981
CCCGCTA37450.019.6674981
TCTTGGA505150.019.2585747
CGTTGAT529800.018.9453931
CGCCGTT55600.018.8318921
CCGCTTT152850.018.282951
CGCGGGT84950.018.1907731
CCCGAAT56950.018.131891
Produced by FastQC (version 0.11.3)