FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI07.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI07.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38739216
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC813020.20987001905252806No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA803640.20744870004596894No Hit
TGACAACCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCA575150.14846712437339982No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT573780.14811347756753776No Hit
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC542960.14015771511741487No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT462850.11947841174689751No Hit
TTTCAGTTCAACTTTAAGCTTACCATCAGAACAACAAATCAAAATGTAAA459420.11859300405046916No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTCATGAATCAACCCGTCTA454340.1172816713688785No Hit
TGAAAATTCATTGTTAGGACCTAAGCCCAATAACGAAAGTAATTCTAATC453670.11710872001126714No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA416930.10762479034165276No Hit
CCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCAACAATA404360.10438001636377979No Hit
CTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAGCTCAACATACATACT402250.1038353486554813No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG401250.10357721230083747No Hit
CCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAG395270.10203355690006737No Hit
CCTAAGCCCAATAACGAAAGTAATTCTAATCATTTATATAATGCACGATA388040.10016723105599247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC251800.043.539681
GTGGCGC241900.035.826463
TGGCGCA209300.033.0846824
TACAACC289700.032.9464652
CTACAAC318550.031.4774231
CGCACGC275800.028.5866891
GGTGGCG375450.027.4347722
GCGCACG255450.027.4279946
GCGACAT97050.025.969337
GGCGCAC280350.025.654665
GCACGCC314150.024.8373952
TGCGACA101200.024.1927666
ACAACCA463200.021.0874773
CGACAAT467200.020.444651
ACCAACC481650.020.3924756
CACGCCT380800.020.3200513
ACGCCTG386550.019.9622844
GCTGCGA132900.018.7401144
GGCCTTA249450.018.6695583
GCCTTAT244500.018.6651524
Produced by FastQC (version 0.11.3)