FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI12.H5VHJBBXX.s_3.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI12.H5VHJBBXX.s_3.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44687347
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTT2427020.5431112301206872No Hit
GTGGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTT1883740.42153766702686557No Hit
CGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTG1417730.3172553519455966No Hit
CTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTT1256920.2812697741935765No Hit
CCGGGTTGTCATACACTTGTGCTAGGAGAATTGGTTCTTGTTACTCATAT1255860.28103257058424164No Hit
CGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAATAATTGGGTCAAT1237000.2768121365540004No Hit
CTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCA1235390.2764518556001993No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA1184470.2650571312725278No Hit
GTTTAACTTTAGTCACTGGGCAGGCAATGCCTCTAATACTTGTTATGCTA1149720.2572808808721628No Hit
CCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCACGCCTGTGTTG1130270.25292841841785774No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCT1099990.2461524511625181No Hit
CTTTTGGCTATATTTTAAGTTTACATTTTGATTTGTTGTTCTGATGGTAA1074100.24035886489300876No Hit
CGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCA1034320.23145701623325277No Hit
GTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTT1020950.2284651178777742No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1018990.22802651497749465No Hit
CAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCTAG1012110.22648692928671732No Hit
ATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGAGTCT990580.22166901069334013No Hit
ATAAGATATTAGTATTACTTTGACTTGTGAGTCTAGGTTAAAATCATTCG969130.21686899425915795No Hit
GTTTTTGGTAAACAGGCGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTT928430.2077612707686585No Hit
GTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTCTCG908630.203330486367875No Hit
GGAATTAGTGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTA809400.18112509565627158No Hit
CTGATGGTAAGCTTAAAGTTGAACTGAAATTCTTTTTTGGGCAACCAGCT806830.18054998879212947No Hit
ATTAGGTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGG801510.1793594952056563No Hit
CCAATTTGTAATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGTG800020.17902606749064784No Hit
GTTGTTTTTATATAAGTTGAACTAGTAAATTAAAGCTCCATAGGGTCTTC796760.17829655450344814No Hit
CTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTGCACCATTGGGAT744140.16652140929288103No Hit
CTTGTTACTCATATTAACAGTATTTCATCTATGGATCTATAGATTAGCCC670040.14993953433843366No Hit
GTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGTGA668700.14963967317191598No Hit
CTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCATTATGCAAAAGG665300.14887883140612487No Hit
CCCAACCGAAATTTTTTAGTTCATATTTATTTTGTTTTAGCCCATTAGGT659030.1474757496792101No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG614500.13751096031724594No Hit
CTCGTTTAGCCATTCATTCTAGTCCCTAATTAAGGAACAAGTGATTATGC579160.12960268149281717No Hit
CCGTTGATCAATAATTGGGTCAATAAGATATTAGTATTACTTTGACTTGT578450.12944379982996082No Hit
GTTAGTACCTATGATTCGATAATTGACAATGGTTATCCGGGTTGTCATAC529950.1185906158179406No Hit
CTCCATTTCTCTTGTCCTTTCGTACTGGGAGAAATTGTAAATAGATAGAA527790.1181072575196733No Hit
GTTCTTGTTACTCATATTAACAGTATTTCATCTATGGATCTATAGATTAG503350.11263814788557486No Hit
GTTCGTGTTTGCCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAAAGCA495020.11077408555938664No Hit
GGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCAT490900.10985212436083977No Hit
GTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTTAAGCCTACAATG490690.10980513119295268No Hit
CGGGGTTCGTGTTTGCCGAGTTCCTTTTACTTTTTTTAATCTTTCCTTAA486160.10879142142853099No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC476770.1066901554930079No Hit
CCCAATTTGTAATATAGGAATTTATTGAGGTTTGTGGAATTAGTGTGTGT475190.10633658784890497No Hit
CCCCAACCGAAATTTTTTAGTTCATATTTATTTTGTTTTAGCCCATTAGG471860.10559141047240955No Hit
CTTAGGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTAT463310.10367811720843485No Hit
TAAGTATGTATGTTGAGCTTGAACGCTTTCTTTATTGATGGCTGCTTTTA459950.1029262265222413No Hit
CTGAAGATGGCGGTATATAGGCTGAATTAGCGAGAAGGGGTAAGGTAGAA458330.10256370779854083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTT102350.038.8729061
GAGTCTT471100.034.976044
GGAGTCT481300.034.294663
AGTCTTG498100.033.992195
CGATTTG99700.033.660342
TGGAGTC495150.033.131772
GTCTTGG522100.032.2225766
CGCCGAG69400.031.7339171
TCTTGGA598000.028.6381667
CCGCTTT120000.028.2211631
CTTGGAA613300.027.4089348
CTGGAGT810900.027.2926981
CCCGTTT105800.026.685441
TTGGAAG658500.025.4182829
CGCCGTT59200.023.7846181
CGCCCAT86250.023.5250471
TCGTTTG862650.023.0994822
CGCTATG89450.023.0063551
CCCGTAT39350.022.754131
CGTTTGG878150.022.7409633
Produced by FastQC (version 0.11.3)