FastQCFastQC Report
Fri 29 Apr 2016
SLX-11274.RPI12.H5VHJBBXX.s_3.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-11274.RPI12.H5VHJBBXX.s_3.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44687347
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAAC2331570.5217517164310516No Hit
CAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAACTCA1731760.387528040096003No Hit
CTTTAATTTACTAGTTCAACTTATATAAAAACAACCTAATGGGCTAAAAC1370270.3066348960031125No Hit
CTGGAATCTCCCAATAAGACGAGAAGACCCTATGGAGCTTTAATTTACTA1337910.29939347260870064No Hit
CATAACTAAACCCCCACATAAACTAAAACATTTAACTCAAAAAGTATTGG1019630.2281697322510553No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT931680.20848854598595887No Hit
CTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAGCTCAACATACATACT923690.20670056783634977No Hit
CCGAAACCAAACGAGCTACCTAAAAACAATTTCATGAATCAACCCGTCTA852910.19086163248849836No Hit
CTAAACCCCCACATAAACTAAAACATTTAACTCAAAAAGTATTGGAGAAA807410.18067977944629382No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTTTAGGTAGAGGTGAAAAGCC785610.1758014410656332No Hit
CTGTCTCTTACTTTCAATCAGTGAAATTGACCTTCCAGTGAAGAGGCTGG757520.169515545418259No Hit
TGACAACCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCA753040.16851302450333425No Hit
CGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCT740130.16562406356322742No Hit
CCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAG702620.157230188670632No Hit
TGAAAAGCCTATCGAGCTTGGTGATAGCTGGTTGCCCAAAAAAGAATTTC702610.15722795090073258No Hit
CTTACACACACACTAATTCCACAAACCTCAATAAATTCCTATATTACAAA687850.15392500252923944No Hit
TTTCAGTTCAACTTTAAGCTTACCATCAGAACAACAAATCAAAATGTAAA636240.1423758720785103No Hit
CAACAAATCAAAATGTAAACTTAAAATATAGCCAAAAGAGGGACAGCTCT613220.1372245257701246No Hit
CCCGGATAACCATTGTCAATTATCGAATCATAGGTACTAACCCAACAATA612990.1371730570624387No Hit
GCAAAATAGTGGGAAGATTTTTAGGTAGAGGTGAAAAGCCTATCGAGCTT609560.13640550198694945No Hit
CTACAATCACTTAACCATTGTAGGCTTAAAAGCAGCCATCAATAAAGAAA604720.13532242135564682No Hit
CGAAACCAAACGAGCTACCTAAAAACAATTTCATGAATCAACCCGTCTAT584150.1307193286726106No Hit
CAATTATCGAATCATAGGTACTAACCCAACAATAGAATTACCTATCCCTA578920.12954897501523194No Hit
CCTCAATAAATTCCTATATTACAAATTGGGCTAATCTATAGATCCATAGA564300.12627735542233018No Hit
GGCTAAACGAGGGTTCAACTGTCTCTTACTTTCAATCAGTGAAATTGACC562810.1259439277073217No Hit
CCTACAACCAACCAACATAACTAAACCCCCACATAAACTAAAACATTTAA547460.1225089509117648No Hit
CACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTG537830.12035397849865645No Hit
CTGGCCTTATAATTAATTGGAGGTAAGATTACACATGCAAACATCCATAA532840.11923733131886302No Hit
TGTAAACTTAAAATATAGCCAAAAGAGGGACAGCTCTTTAGGAAAAGGAA528220.11820348162534688No Hit
CGCCTGTTTACCAAAAACATCACCTCTAGCATAACAAGTATTAGAGGCAT526980.11792599815782306No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGAAGCTATTAATGGTT523830.11722110063951659No Hit
TTTTAACCTAGACTCACAAGTCAAAGTAATACTAATATCTTATTGACCCA519120.11616711101690597No Hit
CAGCTCTTTAGGAAAAGGAAAAAACCTTAAATAGTGAATAAACAACTACA518580.11604627144233914No Hit
CTAAAACAAAATAAATATGAACTAAAAAATTTCGGTTGGGGTGACCTCGG516080.11548682896749274No Hit
CCTGGCCTTATAATTAATTGGAGGTAAGATTACACATGCAAACATCCATA514540.11514221240298736No Hit
TGAAAGACTAATTTAAAGTAAAAACAAGCAAAGATTAAACCTTGTACCTT511480.11445745481377537No Hit
CTTTAGGAAAAGGAAAAAACCTTAAATAGTGAATAAACAACTACAATCAC494410.11063758159552413No Hit
TTTAAGCTTACCATCAGAACAACAAATCAAAATGTAAACTTAAAATATAG488890.10940233261106326No Hit
CACAAACCTCAATAAATTCCTATATTACAAATTGGGCTAATCTATAGATC482400.10795001994636201No Hit
TGAAAATTCATTGTTAGGACCTAAGCCCAATAACGAAAGTAATTCTAATC481650.10778218720390807No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG454250.10165069767959149No Hit
CTTAACCATTGTAGGCTTAAAAGCAGCCATCAATAAAGAAAGCGTTCAAG452290.10121209477931192No Hit
CCTATCCCTAACTCGTTAGCCCAACACAGGCGTGCTTTAAGGAAAGATTA450290.10076454079943478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC332450.071.20751
TACAACC576750.066.870772
CTACAAC595150.065.18811
GTGGCGC312550.054.789163
ACAACCA765500.050.9746133
GGTGGCG437300.048.7887732
TGGCGCA277400.047.928014
GCGCACG313200.042.3735586
ACCAACC938150.041.418186
GGCGCAC377000.035.76815
CCAACCA1114400.034.7577937
GCGCCCG118300.033.8439756
GCGACAT120550.033.8096777
TGCGACA122100.033.085566
CGCACGC400100.032.9542667
GCGGACG74850.032.517436
CGGACGC73300.032.1244437
GCACGCC455100.027.9273478
GGCCTTA318500.027.5269183
TGGCGCC145700.027.4690884
Produced by FastQC (version 0.11.3)