FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC01.HFFYTBBXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC01.HFFYTBBXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41496113
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1301940.31374986857202747No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT917750.2211652932408392No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA651170.15692313157138357No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCT588230.14175544586549588No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG574450.13843465290351412No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA494550.11917983739826427No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA435190.10487488310049667No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG429000.10338317711830021No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT252150.080.5113141
CGATCTG269650.075.0297854
GGCGATC295700.068.649272
GCGATCT353750.057.460783
TGGCGCG433300.042.0757753
GGCGCGT472450.039.1199074
CGGTGGC412750.038.766331
GTGGCGC486200.038.0488322
GGTGGCG516950.035.551831
GCGCGTG552950.033.618825
CGTTTTT287200.032.4867251
CGTGCCT585550.031.305168
GCTCATC476250.030.8243796
TACCGTA108250.029.5974049
CTACCGT111600.028.9670098
GCGTGCC633750.028.9355987
CGCGTGC648900.028.581186
CGACCGT44500.028.2587761
AGCTCAT546350.027.0407375
CATCAGT560150.026.5936349
Produced by FastQC (version 0.11.3)