FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC01.HFFYTBBXX.s_6.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC01.HFFYTBBXX.s_6.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41496113
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1579690.3806838486293885No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC1429850.34457444243030666No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG663160.15981255882930528No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG650820.15683878632198636No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG623740.15031287388291042No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC426480.10277589132264026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTC232150.047.7281
GTCAAAC497550.046.8564347
CAGTCAA558150.041.4335485
CCGTTCT275850.040.2233432
CCCCAGT580600.039.9573752
GAGTCTT647250.039.223914
CCCAGTC606350.038.556673
GGAGTCT670750.038.2575953
ACGTTCG69300.037.194526
AGTCTTG699100.036.5711755
CCAGTCA642500.036.0735174
TGGAGTC750550.034.660662
GTCTTGG760550.034.1750036
GCCCCAG678300.033.8702471
AGTCAAA690850.033.673046
CTTGGAA801200.032.2973028
TCTTGGA858900.030.3958517
CAAACTC780050.030.3486279
CGTTCGA89050.029.187877
CTGGAGT1043200.027.55441
Produced by FastQC (version 0.11.3)