FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC02.HFFYTBBXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC02.HFFYTBBXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39773190
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1011810.25439498315322456No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT681950.17145971947434943No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA619970.15587635791848728No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG574640.14447923337303345No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCT535620.1346686046555481No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA402110.10110076662193804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT200350.075.2465441
CGATCTG216750.069.457964
GGCGATC234850.064.2357562
GCGATCT289750.052.235453
TGGCGCG383400.041.3355753
GGCGCGT389500.040.7409634
CGGTGGC384800.038.313931
GTGGCGC441850.036.3586772
GGTGGCG463200.034.668661
GCGCGTG462450.034.5466085
CGTGCCT521200.030.5010038
CGCGTGC523000.030.4781686
GCGTGCC534600.029.6826137
CGTTTTT279300.029.5758271
CGACCGT37450.028.1749151
TACCGTA96250.027.8266989
CTACCGT99200.027.1443468
ACTACCG106050.025.1873637
GCTCATC412800.024.9232966
CGCGGTG127300.023.8441851
Produced by FastQC (version 0.11.3)