FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC02.HFFYTBBXX.s_6.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC02.HFFYTBBXX.s_6.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39773190
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1222690.30741562343880385No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC1115300.2804150232857862No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG541510.13614950171208293No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG486940.1224292041950872No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG438840.11033563060946332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAAAC457850.041.6726727
CCCGTTC193900.038.4548071
CAGTCAA512900.036.1048775
GAGTCTT558700.035.426754
CCGTTCT215650.034.6901552
CCCCAGT545500.033.9085732
GGAGTCT591400.033.6992343
CCCAGTC560400.032.9295463
AGTCTTG617700.032.298815
CCAGTCA585050.031.4805854
ACGTTCG57250.031.1892456
TGGAGTC655900.030.80282
GTCTTGG661850.030.2966276
AGTCAAA654750.028.8107436
GCCCCAG649950.027.9213451
CTTGGAA724650.027.3729728
CAAACTC735350.026.5046989
TCTTGGA754800.026.3940457
CGTTCGA75900.024.094657
CTGGAGT957600.023.8185231
Produced by FastQC (version 0.11.3)