FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC03.HFFYTBBXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC03.HFFYTBBXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39270800
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT1749420.4454760279902625No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1517330.3863761369770924No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1445450.36807246096336205No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1418260.36114874155861354No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCT650770.165713456308504No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA548240.13960499913421678No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG540350.13759587276042248No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC494170.12583649938376604No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA449110.11436232518818051No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG449100.11435977876692097No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT421660.10737239883068336No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG408880.10411807246096336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT276950.084.376881
GGCGATC288850.080.9457242
CGATCTG305500.076.476754
GCGATCT332950.070.263063
CGGTGGC546900.058.4955181
TGGCGCG663950.047.724564
GTGGCGC718950.044.2870143
GCTCATC631000.043.15256
GGTGGCG738800.042.9829032
CATCAGT677550.040.3900839
GGGAAGC689850.040.213271
GGCGCGT788700.040.202155
AGCTCAT682800.039.963135
GAAGCTC714250.038.3382643
AAGCTCA730650.037.5637934
TCATCAG742500.036.9927258
CGTTTTT294200.036.3366281
CGTGCCT880950.035.9845129
GCGCGTG893050.035.553036
GGAAGCT847250.032.4580272
Produced by FastQC (version 0.11.3)