FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC03.HFFYTBBXX.s_6.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC03.HFFYTBBXX.s_6.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39270800
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1739390.442921967466922No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC1616990.41175377124988544No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG1247020.31754382390987707No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG821040.20907137109506302No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG698860.17795919614573677No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT603610.15370453364841052No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT547340.1393758212208562No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC545850.13899640445318148No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG520260.13248011244996283No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT495210.1261013271947605No Hit
CCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAGG487980.12426026462409728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTC323000.063.033391
CCGTTCT392750.052.651322
GTCAAAC534700.050.050577
CAGTCAA563600.047.024095
CCCCAGT590500.045.0664252
CCCAGTC617200.043.0342643
GGAGTCT664050.042.2316783
GAGTCTT661950.042.093734
AGTCTTG695450.040.313975
CCAGTCA676300.039.3162044
AGTCAAA693450.038.4057126
TGGAGTC740650.038.1953122
GCCCCAG695750.037.971581
GTCTTGG765000.037.3830266
CAAACTC758400.035.8571139
CTTGGAA793050.035.8065958
ACGTTCG80850.034.7305156
TCTTGGA877750.032.5072257
CTGGAGT1020750.030.2858751
TCAAACT909000.029.9639558