FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC04.HFFYTBBXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC04.HFFYTBBXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35763782
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT1670720.467154172900394No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1529470.42765890922833605No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1240670.3469068232213249No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG955840.26726479878442383No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA516470.1444114607342143No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA499140.13956577634882128No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG462120.12921452211066492No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG461670.12908869649188667No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCT410530.11478931394895539No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC396190.11077967089722221No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG389250.10883916024317561No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT381420.1066497944764343No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC372350.10411370922683737No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT362190.10127284636731093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT271700.087.622851
GGCGATC290650.082.0555952
CGATCTG312700.076.11984
GCGATCT330650.072.014693
TGGCGCG561750.048.2460753
CGGTGGC464800.047.2550281
GCTCATC581200.045.8092966
GTGGCGC609050.044.940012
CATCAGT620150.043.0024539
GGTGGCG635600.042.8232731
GGCGCGT640400.042.564934
AGCTCAT627350.042.519745
GGGAAGC636450.042.2211
TCATCAG680200.039.39668
AAGCTCA680050.039.246814
GAAGCTC682100.039.0051423
GCGCGTG749000.036.4693345
CGTGCCT781150.034.8859448
GGAAGCT777550.034.4140362
GCGTGCC844700.032.261347
Produced by FastQC (version 0.11.3)