FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC04.HFFYTBBXX.s_6.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC04.HFFYTBBXX.s_6.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35763782
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1978670.5532608380176347No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG1011180.28273855376928536No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC722000.20188021501752806No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG685070.19155412590312737No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT502450.14049129367805674No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT462310.12926764848303796No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC459800.12856582114274157No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG451940.126368067001415No Hit
CCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAGG420360.11753790468804447No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT400970.11211621858113328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTC286500.058.9907151
CCGTTCT345000.049.4621122
GGAGTCT645250.048.8072663
GAGTCTT653300.047.8531844
AGTCTTG686350.045.673955
TGGAGTC719100.044.176332
GTCTTGG766750.041.354296
CTTGGAA766900.041.3086438
TCTTGGA858950.036.9488337
CTGGAGT964450.035.555561
GTCAAAC355050.034.4735837
ACGTTCG67800.033.2385186
CAGTCAA382800.031.4289845
TTGGAAG1028500.031.0187649
CCCCAGT405500.029.8661042
CCCAGTC438650.027.7069033
CCCCGTT153650.025.9751171
GTTCTCT671500.025.3582254
AGTCAAA499000.024.4421776
CCAGTCA501050.024.0838894
Produced by FastQC (version 0.11.3)