FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC05.HFFYTBBXX.s_6.r_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC05.HFFYTBBXX.s_6.r_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34764314
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT1632000.46944691616811424No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1557490.4480140180531105No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1339290.3852485051193589No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1295580.37267526694184155No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCT853310.24545572796287593No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA612140.17608286474457688No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG560530.1612371813233536No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT504100.14500501865217305No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT498010.14325322225544276No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG494210.14216014732809054No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA445250.1280767398430471No Hit
TCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTG397020.11420331780457396No Hit
AGGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCAC358430.10310285426601543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT229850.088.6321951
GGCGATC259700.078.8289262
CGATCTG277950.073.617844
GCGATCT295550.069.35973
CGGTGGC513550.054.88111
TGGCGCG657800.051.21653
GTGGCGC702000.048.179712
GGTGGCG719650.046.9161571
GGCGCGT746500.045.330844
GCTCATC552650.044.97126
CGTTTTT306350.044.9148561
CATCAGT598100.041.602689
AGCTCAT611450.040.7054255
GGGAAGC619100.040.540111
GCGCGTG853000.039.6869285
CGCGGTG155950.039.157261
AAGCTCA654450.038.152974
TCATCAG653200.038.148448
GAAGCTC653050.038.0384983
CGTGCCT918850.036.6944928
Produced by FastQC (version 0.11.3)