FastQCFastQC Report
Thu 11 Aug 2016
SLX-12161.L2V18DRBC05.HFFYTBBXX.s_6.r_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSLX-12161.L2V18DRBC05.HFFYTBBXX.s_6.r_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34764314
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC1690650.48631766471790583No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1592630.45812208461815185No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG1028770.2959270244768817No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG868550.24983953372415174No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG800670.2303137637060809No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT627460.18048968260958637No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG584340.16808615869710533No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT577820.16621067224280622No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT504570.1451402147616087No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG431360.12408126333227802No Hit
CCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAGG428440.12324132154599686No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT376730.1083668729951064No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC358560.10314024893458273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTC275350.059.5991061
GTCAAAC535700.050.9518747
CCGTTCT331900.050.6546062
CCCCAGT561050.048.0349732
CAGTCAA567550.047.5089155
GGAGTCT578450.046.253723
GAGTCTT584100.045.683044
CCCAGTC610600.044.183863
AGTCTTG616500.043.4097145
TGGAGTC636750.042.4261052
GCCCCAG645850.041.5271841
CCAGTCA652400.041.419174
GTCTTGG668450.040.5638776
AGTCAAA690350.039.1623426
CTTGGAA699300.038.362548
CAAACTC733550.037.601969
TCTTGGA768500.035.133027
CTGGAGT933100.031.7387941
TCAAACT868350.031.6486668
ACGTTCG51100.030.0114356
Produced by FastQC (version 0.11.3)