FastQCFastQC Report
Thu 23 Jun 2016
A2604_OPC_3_May.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameA2604_OPC_3_May.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150042284
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG4812640.3207522487460935No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1676130.11171050955209398No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1558490.10387005305784335No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1543400.10286433656261856No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG1517070.10110949790660345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTT2016200.038.5197874
AGTCTTG2089250.037.340135
GGAGTCT2106150.037.234213
CGAGATT740950.034.665138
TGGAGTC2284750.034.523522
CGCGAGA745200.034.1971556
GTCTTGG2439100.032.9137156
GCGCGAG797300.032.395795
CTTGGAA2451300.031.9159838
TTGGAAG2626350.030.1614069
TCTTGGA2751650.028.9868537
GTGCGTA709100.028.5935461
GCGTACT667450.028.4724123
CTGGAGT3065750.027.2823281
TGCGTAC788350.024.9290162
CGTACTT809350.024.5922934
GGCGCGA1067400.024.0845414
GCGGATA371000.022.42446
CGGCGCG1225800.021.1194653
CGGATAT396950.021.0853527
Produced by FastQC (version 0.11.5)