FastQCFastQC Report
Thu 23 Jun 2016
OPCY1404_19_Apr.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOPCY1404_19_Apr.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148479623
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG4559340.30706839820033754No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT3486410.23480730416455867No Hit
CCCATGCATACCATATAGTAAACCCAAGCCCATGACCACTAACAGGAGCC1774560.11951538966394062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC678300.080.097832
GCGATCT723750.075.019583
TGGCGCA1362100.065.403394
CGATCTG837250.065.11634
GGGCGAT889700.061.1012341
GCGCACG1603400.055.5987476
GTGGCGC1656950.053.8736573
CGCACGC1735300.051.7378967
CACGCCT1792050.049.75299
CGGTGGC1850950.048.7714081
GCACGCC1862550.047.9714748
GGTGGCG1982600.045.0700722
GGCGCAC2263800.039.6049235
CGTTGAC510400.036.473461
AACGCGT111100.028.3523868
ACCGTTG353250.028.290563
GCATACC1043000.026.1048726
TACCATA1063650.025.7483929
AACCGTT395200.025.4185712
TGCGGAA793800.025.415231
Produced by FastQC (version 0.11.5)