FastQCFastQC Report
Thu 23 Jun 2016
OPCY1404_19_Apr.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOPCY1404_19_Apr.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148479623
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG4959540.3340215916361802No Hit
CTCCTCTATTCGGGGAAGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGG1935840.13037748620899986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTT2019300.039.1209144
GGAGTCT2161300.036.9777153
AGTCTTG2151900.036.9386525
TGGAGTC2294950.034.9792182
GTCTTGG2412550.033.63996
CTTGGAA2545650.031.4992778
TTGGAAG2677650.030.317369
TCTTGGA2668550.030.2482367
CTGGAGT3008400.028.2073421
TAGCCGA383300.027.3167384
CGCGAGA509250.026.9216296
GCGTACT567500.026.5591223
CGAGATT557250.025.0158738
GTGCGTA651400.024.7593671
GCGCGAG601800.023.2597395
TGCGTAC679750.023.0957782
CGTACTT688150.022.8859484
CGACATC476200.022.3216958
AGCCGAC474800.022.1753775
CCGACAT489200.021.552057
Produced by FastQC (version 0.11.5)