FastQCFastQC Report
Thu 23 Jun 2016
OPC_0605A_10_May.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOPC_0605A_10_May.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116092023
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG1928910.16615353494184523No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1401890.12075678963747577No Hit
CCCATGCATACCATATAGTAAACCCAAGCCCATGACCACTAACAGGAGCC1351490.1164154060783315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC348150.062.968112
GCGATCT366850.059.5100443
TGGCGCA711200.053.769274
CGATCTG449100.049.316574
GCGCACG820050.046.4865846
GGGCGAT491900.044.797261
CGCACGC908750.042.2424967
GTGGCGC939350.040.6867563
CACGCCT944300.040.4103939
GCACGCC995600.038.5864148
CGTTGAC386700.034.3696171
CGGTGGC1186800.032.866821
GGTGGCG1218500.031.48472
GGCGCAC1249100.030.824175
GCATACC795800.025.5103916
TACCATA827300.024.9925569
ACCGTTG262000.024.349053
TGCGGAA603650.022.8898431
CGCGAGT215950.022.6869341
ATGCATA914500.022.3921364
Produced by FastQC (version 0.11.5)