FastQCFastQC Report
Thu 23 Jun 2016
OPC_0605A_10_May.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOPC_0605A_10_May.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116092023
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2092370.18023374439775247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGA457300.030.1528536
GTGCGTA468000.029.7645241
CGAGATT480500.028.9665188
GCGTACT485250.027.3209823
GCGCGAG515350.027.1613585
TGCGTAC516650.026.1632562
GAGTCTT1337050.025.5881964
GGAGTCT1408200.024.6331753
AGTCTTG1407350.024.617175
CGTACTT588600.023.9426844
TGGAGTC1552400.022.4850232
GTCTTGG1646900.021.9628856
TAGCCGA261700.020.8027324
GCGGATA257900.020.6776436
CTTGGAA1680750.020.4843048
TTGGAAG1758000.020.079529
GGCGCGA708650.019.754534
CGGATAT277050.019.4302147
TCTTGGA1885100.019.0578697
CTATTCG616750.018.284966
Produced by FastQC (version 0.11.5)