FastQCFastQC Report
Thu 23 Jun 2016
OPC_0605Y_10_May.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOPC_0605Y_10_May.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127962571
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG3941570.30802522715802577No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1911120.1493499220174312No Hit
CCCATGCATACCATATAGTAAACCCAAGCCCATGACCACTAACAGGAGCC1769280.13826543075631076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC444550.066.898292
TGGCGCA1155750.064.708254
GCGATCT457250.064.6702353
CGCACGC1392650.053.915927
GCGCACG1388650.053.8894426
CGATCTG560400.053.1906974
GTGGCGC1425250.052.5635263
CACGCCT1484350.050.4616289
GCACGCC1500700.049.9573178
GGGCGAT603550.049.400031
CGGTGGC1633900.046.640121
GGTGGCG1772100.042.451822
GGCGCAC1919100.039.3051225
CGTTGAC464800.036.3891261
TACCATA968550.028.958829
ACCGTTG316350.028.7681963
GCATACC977050.028.580076
ATGCATA1011150.027.8273854
AACCGTT334300.027.4389652
CATACCA1137800.024.9153847
Produced by FastQC (version 0.11.5)