FastQCFastQC Report
Thu 23 Jun 2016
OPC_0605Y_10_May.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOPC_0605Y_10_May.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127962571
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2773750.21676260318339494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA338950.032.3995254
GTGCGTA524950.031.2745571
GAGTCTT1518600.029.561594
AGTCTTG1608700.028.1704065
TGCGTAC569550.028.0197032
GCGTACT560000.027.8535963
GGAGTCT1652100.027.5480963
CGACATC431700.025.7394088
TGGAGTC1793900.025.4516932
GTCTTGG1870450.024.9592936
CCGACAT441150.024.9434017
AGCCGAC443400.024.8817525
CGTACTT653650.024.6998754
CGCGAGA498200.024.1961126
CGAGATT513750.023.5757548
CTTGGAA1936350.023.4668088
GCCGACA473550.023.2215926
GACATCG481800.023.1823929
TTGGAAG2026700.022.75099
TCTTGGA2119450.021.891177
Produced by FastQC (version 0.11.5)