FastQCFastQC Report
Thu 23 Jun 2016
OPC_A1404_19_Apr.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOPC_A1404_19_Apr.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114932173
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG2697650.23471669677732449No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT2170870.1888827073686321No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC2043650.17781357009581641No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1281400.11149184484661226No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1197400.10418318637375802No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGCTCCCCCCC1162450.10114226240201687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA409500.042.766624
CGACATC491000.035.8663948
CCGACAT501750.034.897277
AGCCGAC508650.034.6078155
GACATCG528450.033.4880529
GCCGACA534800.032.9559756
CGCGAGA647350.028.7269886
CGAGATT675700.027.649428
GCGCGAG686100.027.4296955
GAGTCTT1581750.027.2153154
GTAGCCG655950.027.1051453
GTACAAA1283800.026.3697936
AGTCTTG1663500.026.1220385
GGAGTCT1667700.026.0894033
GTGTACA1363950.025.5899754
GTCTGGA1676650.025.4139351
GCGGATA320900.024.3554886
TGGAGTC1815750.024.0859262
GTCTTGG1899850.023.9519376
TGTGTAC1444700.023.875873
Produced by FastQC (version 0.11.5)