FastQCFastQC Report
Thu 23 Jun 2016
Y2604_OPC_3_May.r_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameY2604_OPC_3_May.r_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121051011
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG3668760.3030755356516601No Hit
CCCATGCATACCATATAGTAAACCCAAGCCCATGACCACTAACAGGAGCC2025550.16733028359424443No Hit
ACTAGACACATCCACATGATTTATTACAATCATCTCCTCAATAGCCACAC1423690.11761074841415409No Hit
CGTTGACTCTTTTCAACTAACCACAAAGATATCGGAACCCTCTACCTATT1226140.10129118211164713No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT1212060.1001280361053738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA1031250.064.149194
GGCGATC334000.056.153712
GCGCACG1182950.055.8751876
CGCACGC1208950.054.7931027
GCGATCT346350.054.0495763
GTGGCGC1294950.051.275073
GCACGCC1378100.048.015538
CACGCCT1376250.047.983099
CGGTGGC1487800.045.1686361
CGATCTG430500.043.9193274
GGTGGCG1586100.041.980142
GGGCGAT489850.038.3992921
CGTTGAC517600.037.4975851
ACTAGAC630950.035.7927171
GGCGCAC1949350.034.2727135
ATGCATA980000.032.6425744
ACCGTTG332200.032.337273
AACCGTT338700.031.9132482
GCATACC1037700.030.5625326
TACCATA1045050.030.4663689
Produced by FastQC (version 0.11.5)