FastQCFastQC Report
Thu 23 Jun 2016
Y2604_OPC_3_May.r_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameY2604_OPC_3_May.r_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121051011
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1519020.1254859407989579No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA316750.027.2576944
GTGCGTA448550.026.5861931
TGCGTAC467450.024.5870172
CGACATC363550.024.3686498
GCGTACT483000.023.6604633
CCGACAT372850.023.6450777
CGCGAGA414250.023.5926156
AGCCGAC375850.023.015945
GACATCG412400.021.5693659
CGTACTT545250.021.5399594
GCCGACA410450.021.2861566
CGAGATT474400.020.9652928
GCGCGAG479650.020.8409065
GATTAAC1004800.019.4244566
GAGTCTT1255400.019.3243774
AGTCTTG1317250.018.6960815
TAGCTTA736850.017.7953629
GGAGTCT1391250.017.7637843
GCGGATA271150.017.650256
CGGATAT280750.017.3030577
Produced by FastQC (version 0.11.5)